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KMID : 0379119880160040247
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Korean Journal of Mycology
1988 Volume.16 No. 4 p.247 ~ p.252
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Uptake of Isolated Lyophyllum ulmarium Chromosomes by Ganoderma applanatum Protoplasts
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Abstract
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The uptake of isolated Lyophyllum ulmarium chromosomes by Ganoderma applanatum protoplasts was induced with polyethylen glycol+CaCl₂+glycine. Uptake products of chromosomes by protoplasts showed micro-and macrotransgenome type. The former was slowly growing and unstable, the latter was outgrowing and stable mycelial colonies which made thick hyphae of width and segregation of mycelial colony on GCM containing benomyl. A comparison of macrotransgenome type was using isozyme analysis of esterase. The enzyme pattern of two transformants was distinct in position and quantity compared with parents.
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